Anti Protein S (human)
Mouse monoclonal antibody
Cat.No. HYB 232-11
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Protein S is a vitamin K-dependent, potent neutral anticoagulant plasma glycoprotein. Protein S has a molecular weight of approximately 70 kDa and consists of a single polypeptide chain. The concentration of protein S in human plasma is 25 µg/ml, with a half-life of approximately two days. About 40% of protein S in plasma is in a free form, wheras 60% is complexed with C4b-binding Protein (C4BP). Only the free protein S functions as a cofactor to antigen presenting cells (APC) in the APC-dependent degradation of Factor Va and Factor VIIIa.
Protein S isolated from human plasma
HYB 232-11 has specificity for human protein S
Epitope specificity differs from that of HYB 232-02
HYB 232-11 reacts strongly with protein S. Strong reaction is seen in sandwich ELISA in combination with a biotinlabeled antibody against protein S. Reactivity is Ca++ dependent and is optimal when using a tris-buffer containing 1 mM CaCl2. In Dot blotting SDS-treatment abolishes the reactivity.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:20,000 | |
| Immunoblotting | No | ||
| Immunohistochemistry | Not determined |
1. Law SKA, Reid KBM (1988) Complement. In: In Focus (Ed. Male D) IRL Press: Oxford.
2. Bendtzen K et al (2000) Basal og klinisk immunologi. FADL´s Forlag A/S, Copenhagen.
